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KMID : 1159320100120020127
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Korean Journal of Vision Science
2010 Volume.12 No. 2 p.127 ~ p.138
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Evaluation of Lipids adsorbed on Silicone Hydrogel Contact Lenses using High Performance Liquid chromatography Analytical Method
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Kang You-Sun
Lee Koon-Ja
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Abstract
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Purposes: The purpose of this study is to investigate and compare the in vitro sorption of major tear film lipids such as oleic acid, oleic acid methyl ester and cholesterol on the silicone hydrogel materials.
Methods: Lipid spoilation was performed by incubating a lotrafilcon A, lotrafilcon B, galyfilcon A and balafilcon A silicon hydrogel lenses, polymacon soft contact lenses and RGP lenses in phosphate buffered saline (PBS) containing oleic acid, oleic acid methyl ester, and cholesterol at 37℃ for 24 hours with shaking. Lipids were extracted from the lenses with a solvent containing 1 : 1 methanol/chloroform (v/v) for 4 hours. High performance liquid chromatography methods using C-18 column were used to analyze lipid deposit extracts from contact lenses.
Results: The amount of total lipids on silicone hydrogel contact lenses were 48~67 ㎍ per lens depending on the silicone hydrogel lens materials versus 18 and 177 ㎍ per lens for polymacon and RGP contact lenses, respectively that showed 300~372% and 30.5~37.8% of that of polymacon soft contact lenses and RGP lenses, respectively. After 24 hours of soaking, galyfilcon A showed the highest adsorption of all the silicone hydrogel lenses (p < 0.001) and there is no statistically different among balafilcon A, lotrafilcon A and lotrafilcon B materials in lipid sorption. Among the three lipid compounds, oleic acid is the most prevalently sorbed on the silicone hydrogel lenses, polymacon soft contact lenses and RGP lenses.
Conclusions: In vitro lipid sorption varied depending on the lens material and the quantity of lipid adsorption by silicone hydrogels were higher than polymacon soft lenses and lower than RGP lenses and the results get in this study will be cornerstones in the development of cleaners for silicone hydrogel lenses.
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KEYWORD
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high-performance Liquid chromatography, silicone hydrogel Lens, Lipid deposit
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